But the group continues. In 2018, Women's March leaders helped rally against Trump's Supreme Court nominee Brett Kavanaugh as he faced questions over sexual assault allegations.
Then, in 2020, they held a vigil for the late Supreme Court Justice Ruth Bader Ginsburg, known for her work on gender equality.
In 2022, when the Supreme Court ultimately struck down federal abortion rights, Women's March organizers launched a "summer of rage" with protests held across the country.
But the organization also continues to weather controversy over its membership.
In 2018, for example, a founding member claimed she was pushed out of a leadership position because of her Jewish beliefs. Outcry over anti-Semitism led to the ouster of other leaders. Critics also accuse the organization of excluding people of color and whitewashing feminism.
By 2019, the movement's attendance was much smaller than previous annual marches, disappointing some attendees.
The organization has since appointed new leadership, such as Tamika Middleton, who has served as managing director since 2021. She acknowledged that the organization has had to evolve to keep up with the times.
"I think we're always learning, I think we're always practicing, right?" she said. “Our values don’t always translate into our practices in the way we want them to.”
Middleton, who describes herself as part of the "black radical tradition in the South," told Al Jazeera that this year's annual protest - dubbed the People's March - would not attempt to recreate the massive momentum of 2017.
Instead, she hopes Tuesday's People's March will bring together a broader coalition of activists interested in promoting the rights of immigrants, LGBTQ+ people, the poor, and women.
"We're recognizing the connections between all of these battles, that there are threats and opposition that extend beyond Trump," Middleton said.
The shifting tide within the movement was already apparent last November when the Women's March helped organize an impromptu protest outside the conservative think tank The Heritage Foundation.
It was the weekend after the 2024 election, and Middleton noticed a difference in how protesters reacted to Trump's latest victory.
"When Trump was first elected, this anger really grew rapidly," she explains. "What we're seeing this time is, yes, we're seeing some anger. We're also seeing frustration, we're seeing disappointment, we're seeing sadness. We're seeing a lot of sadness."
For Marie, an activist who marched in San Francisco in 2017, the past four years of Democratic President Joe Biden have also contributed to a change in public sentiment.
Under Biden, the United States continues to provide unconditional military aid to its ally Israel, even as the Middle Eastern country launches a devastating 15-month war against Gaza that has killed more than 46,800 Palestinians. UN experts found Israel's tactics in the enclave "consistent with genocide."
Mary explained that she believes recent events are part of a "legacy of violence" that transcends party lines.
"Trump is not a monster," Mary said. "This country prioritizes bombing, especially bombing children, rather than educating them."
She added that political change requires more sustained activism than annual protests.
"The action required to change government is not a few signs in a few hours on Saturday," Mary said. "We have left the realm of lovely protests."