The interview took place less than a week before Trump returned to power after taking a tough stance against Tehran.
Iranian President Masoud Pezeshkian has denied claims that Iran made two attempts to assassinate US President-elect Donald Trump in 2024.
In an interview with US media NBC News broadcast on Tuesday, Pezeshkian also insisted that Iran's nuclear program was for peaceful purposes and warned Trump not to risk "war."
The interview with Iran's president, who is widely seen as centrist, aired less than a week before the inauguration of Trump, who implemented hardline policies toward Iran during his first term.
"We have never tried (to assassinate Trump) in the first place and we never will," Pezeshkian told NBC.
In November, the U.S. Department of Justice charged an Iranian man in connection with a plot by Iran's elite Islamic Revolutionary Guard Corps (IRGC) to assassinate Republican leaders. Law enforcement foiled the alleged plan before any attack could be carried out.
During his US campaign last year, Trump suggested that Iran might be behind his assassination.
The 78-year-old, who won last year's US election and will take office on Monday, suffered two assassinations during his campaign - one in September while playing golf at a golf course in West Palm Beach, Florida, and another One was an assassination in July. Rally in Butler, Pennsylvania.
Investigators found no evidence of Iranian involvement. Iran has also previously denied U.S. claims of interfering in U.S. affairs, including through cyber operations.
Pezeshkian also reiterated that Iran's nuclear program is for peaceful purposes and Tehran does not seek "nuclear weapons."
During Trump's first term, the United States withdrew from the 2015 Joint Comprehensive Plan of Action (JCPOA), which lifted sanctions on Iran's nuclear program in exchange for oversight.
Trump also threatened during his recent presidential campaign that U.S. ally Israel could attack Iran's nuclear facilities.
"I do hope that Trump will promote peace in the region and the world and not in turn encourage bloodshed or war," Pezeshkian told NBC. "We will respond to any action. We are not afraid War, but we don’t seek war either.”
Asked about the possibility of talks with Trump, the Iranian president said: "The problem we face is not dialogue. What we have to commit to is the commitments that come out of negotiations and dialogue... It is the other side that is not living up to its commitments and obligations. "
Iranian Foreign Minister Abbas Araghchi reported on Tuesday that ongoing talks with European powers showed they were serious about resuming talks on its nuclear program.